PXD023059

PXD023059 is an original dataset announced via ProteomeXchange.

Title	Rapid and high coverage profile of human cysteineome
Description	Cysteines play important roles in many biological activities, including protein folding, aging, signaling and etc. To identify and quantitatively profile the oxidation and reactivity of cysteines, cysteine-reactive compounds have been applied to covalently label and enrich the cysteines. And with mass spectrometry (MS), thousands of cysteines can be profiled at the same time with decoration of functional groups. However, with all the technology and methods developed to characterize cysteines, current datasets have only uncovered about 5% of the total cysteinome. Here we show a comprehensive and high coverage profile of more than 30,000 cysteines. With incorporation of new techniques - single-pot, solid phase-enhanced sample-preparation (SP3) and High-field asymmetric waveform ion mobility spectrometry (FAIMS), we have successfully identified as many as 14,306 cystienes in a single-shot experiment. With cysteine profiles in different cell lines, proteases and fractionations, our results have successfully expanded the cysteine library to a great extent.
HostingRepository	PRIDE
AnnounceDate	2021-02-04
AnnouncementXML	Submission_2021-02-03_23:21:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianyang Yan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-10 23:55:03	ID requested
1	2021-02-03 15:19:20	announced
⏵ 2	2021-02-03 23:21:28	announced	2021-02-04: Updated project metadata.

10.1002/CBIC.202000870;

Yan T, Desai HS, Boatner LM, Yen SL, Cao J, Palafox MF, Jami-Alahmadi Y, Backus KM, SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem, 22(10):1841-1851(2021) [pubmed]

submitter keyword: Cysteine, Proteomics, SP3, FAIMS, LC-MS/MS

Keriann M. Backus
contact affiliation	Biological Chemistry Department David Greffen School of Medicine UCLA
contact email	kbackus@mednet.ucla.edu
lab head
Tianyang Yan
contact affiliation	Department of Chemistry and Biochemistry, UCLA
contact email	tyyan14@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD023059

PRIDE project URI

• PRIDE
  1. PXD023059
     1. Label: PRIDE project
     2. Name: Rapid and high coverage profile of human cysteineome