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PXD023037 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome profile of myelin in the zebrafish brain
DescriptionWhile the myelin proteome is well characterized in mice, much less is known about the protein composition of myelin in non-mammalian species such as zebrafish (Danio rerio), despite its increasing popularity as model organism for myelin biology. Here, we assess the proteome of myelin biochemically purified from the brains of adult zebrafish by complementary proteomic approaches for deep qualitative and quantitative coverage. A data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) provided the high dynamic range required for correct quantification of highest-abundance proteins. According to this analysis, the predominant Ig-CAM myelin protein zero (MPZ/P0), myelin basic protein (MBP) and the short chain dehydrogenase 36K constitute 12%, 8% and 6% of the total myelin protein, respectively. Combined with data from an ion mobility-enhanced version of the MSE mode (referred to as UDMSE) and from 1D-gel-based proteomic approaches, we provide the most comprehensive proteome resource of zebrafish myelin so far, correlate it with previously established mRNA-abundance profiles, and demonstrate both similarities and heterogeneity of myelin composition between teleost fish and rodents.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlaf Jahn
SpeciesList scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-10 00:50:05ID requested
12021-03-22 03:36:50announced
22022-03-02 06:54:11announced2022-03-02: Updated publication reference for PubMed record(s): 33898427.
Publication List
Siems SB, Jahn O, Hoodless LJ, Jung RB, Hesse D, M, ö, bius W, Czopka T, Werner HB, Proteome Profile of Myelin in the Zebrafish Brain. Front Cell Dev Biol, 9():640169(2021) [pubmed]
Keyword List
submitter keyword: Oligodendrocyte, myelin proteome, central nervous system (CNS), label-free proteomics, transcriptome, PLP, MBP, MPZ, CD59, teleost, zebrafish, danio rerio
Contact List
Olaf Jahn
contact affiliationProteomics Group Max-Planck-Institute of Experimental Medicine Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact emailjahn@em.mpg.de
lab head
Olaf Jahn
contact affiliationProteomics Group, Max-Planck-Institute of Experimental Medicine, Göttingen
contact emailjahn@em.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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