Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults and its overall incidence has been increasing gradually over the years. Extracellular vesicles (EVs) are known to express the properties of their parental cells, including proteins, RNAs, and DNAs. As EVs are representative of their parental tissues, they are excellent tools for understanding how cancer cells adapt to their environment. Proteomic analysis is a useful technique in cancer research that can be both quantitative and qualitative when determining the interrelationships between proteins in cells. It is clinically important to identify novel differentially expressed proteins (DEPs) from EVs in AML that could be used as biomarkers