PXD022652 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A WDR35-dependent coatomer transports ciliary membrane proteins from the Golgi to the cilia |
Description | Intraflagellar transport (IFT) is a highly conserved mechanism for motor-driven transport of cargo inside cilia. However, the mechanism of selective transport of cargo to cilia and entry across the diffusion barrier is not well understood. WDR35/IFT121 is a component of the IFT-A complex, best known for ciliary retrograde transport. Small Wdr35 mutant cilia are formed but fail to enrich in diverse classes of ciliary membrane proteins. Whilst the assembly of the IFT-B complex is unaffected, these exhibit retrograde trafficking defects in Wdr35 mutants. In contrast, the IFT-A peripheral components are degraded and core components remain stuck at the cilia base. The deep sequence homology and structural similarity of WDR35 and other IFT-As to the coatomer COPI proteins α and ß’, and accumulation of electron-light vesicles around Wdr35 mutant cilia, suggest that WDR35 is required to form coated vesicles delivering cargo from the Golgi necessary for cilia elongation. This is the first insitu proof towards a novel coatomer function for WDR35 and likely other IFT-A proteins in transporting ciliary membrane proteins from the Golgi. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-03 |
AnnouncementXML | Submission_2021-05-02_22:11:36.692.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jimi Wills |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-20 07:35:01 | ID requested | |
⏵ 1 | 2021-05-02 22:11:37 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cilia, WDR35, IFTA, ciliary membrane |
Contact List
Pleasantine Mill |
contact affiliation | MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU |
contact email | pleasantine.mill@igmm.ed.ac.uk |
lab head | |
Jimi Wills |
contact affiliation | University of Edinburgh |
contact email | jimi.wills@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022652
- Label: PRIDE project
- Name: A WDR35-dependent coatomer transports ciliary membrane proteins from the Golgi to the cilia