PXD022639
PXD022639 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted mass spectrometry quantification of total soy protein residues from commercially processed ingredients for food allergen management |
Description | Soybean is one of the most commonly allergenic foods in the U.S., and the ubiquity and variety of commercial soy ingredients in the food industry make it a challenging allergen to avoid. The processing methods used to produce soy-derived ingredients result in protein modifications that often substantially impact detection and quantification with commonly used antibody-based methods. This study aimed to develop a mass spectrometry (MS)-based method capable of quantifying commercially processed soy ingredients in food matrices. A quantification strategy using external standards with internal calibrants was developed and evaluated, resulting in the ultimate use of a matrix-independent standard curve of non-roasted soy flour with milk proteins as carrier proteins. The method performance was evaluated by quantifying six soy-derived ingredients in incurred food matrices. Out of the twelve ingredient-matrix combinations with 10 ppm incurred total soy protein, eight had maximum recoveries between 60-120%. Compared with three commercial ELISA kits, the MS method showed a substantial advantage in quantifying the highly processed soy proteins in food matrices. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2021-03-29 |
AnnouncementXML | Submission_2021-03-29_20:25:55.159.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Shimin Chen |
SpeciesList | scientific name: Glycine max; NCBI TaxID: 3847; |
ModificationList | Label:13C(6)15N(2); Label:13C(6)15N(4) |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-11-19 12:13:33 | ID requested | |
⏵ 1 | 2021-03-29 20:25:55 | announced |
Publication List
Chen S, Yang C, Downs M, Targeted mass spectrometry quantification of total soy protein residues from commercially processed ingredients for food allergen management. J Proteomics, 239():104194(2021) [pubmed] |
Keyword List
submitter keyword: Soybean |
food allergen |
quantification |
LC-MS/MS |
mass spectrometry |
Contact List
Melanie Downs | |
---|---|
contact affiliation | University of Nebraska-Lincoln |
contact email | mdowns2@unl.edu |
lab head | |
Shimin Chen | |
contact affiliation | University of Nebraska-Lincoln |
contact email | shimin@huskers.unl.edu |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |