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PXD022553 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMicro cell surface proteomics myeloma
DescriptionThe myeloma cell surface proteome (“surfaceome”) not only determines tumor interaction with the microenvironment but serves as an emerging arena for therapeutic development. Here, we use glycoprotein capture proteomics to first define surface markers most-enriched on myeloma when compared to B-cell malignancy models, revealing unexpected biological signatures unique to malignant plasma cells. We next integrate our proteomic dataset with existing transcriptome databases, nominating CCR10 and TXNDC11 as possible monotherapeutic targets and CD48 as a promising co-target for increasing avidity of BCMA-directed cellular therapies. We further identify potential biomarkers of resistance to proteasome inhibitors and lenalidomide including changes in CD53, EVI2B, CD10, and CD33. Comparison of short-term treatment with chronic resistance delineates large differences in surface proteome profile under each type of drug exposure. Finally, we develop a miniaturized version of the surface proteomics protocol and present the first surface proteomic profile of a primary MM patient plasma cell sample. Our dataset provides a unique resource to advance the biological, therapeutic, and diagnostic understanding of myeloma.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIan Ferguson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-16 03:39:28ID requested
12022-05-26 19:28:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Multiple Myeloma, Plasma Cell, Cell Surface
Contact List
Arun P. Wiita
contact affiliationDept. of Laboratory Medicine, University of California, San Francisco, CA
contact emailarun.wiita@ucsf.edu
lab head
Ian Ferguson
contact affiliationStanford University
contact emailifergus@stanford.edu
dataset submitter
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Dataset FTP location
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