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PXD022252 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDual DNA/protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia
DescriptionThe architecture of chromatin specifies eukaryotic cell identity by controlling transcription factor access to sites of gene regulation. Here we describe a dual transposase/peroxidase approach, integrative DNA And Protein Tagging (iDAPT), which detects both DNA (iDAPT-seq) and protein (iDAPT-MS) associated with accessible regions of chromatin. In addition to direct identification of bound transcription factors, iDAPT enables the inference of their gene regulatory networks, protein interactors, and regulation of chromatin accessibility. We applied iDAPT to profile the epigenomic consequences of granulocytic differentiation of acute promyelocytic leukemia, yielding previously undescribed mechanistic insights with potential therapeutic implications. Our findings demonstrate the power of iDAPT as a discovery platform for both the dynamic epigenomic landscapes and their transcription factor components associated with biological phenomena and disease.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathan Lee
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListbiotinylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-29 08:28:39ID requested
12020-11-01 22:47:41announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human, Hematopoietic cancer cells, TMT, iDAPT
Contact List
Jonathan Lee
contact affiliationCancer Research Institute, Beth Israel Deaconess Cancer Center Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA.
contact emailjdlee@post.harvard.edu
lab head
Jonathan Lee
contact affiliationHarvard University
contact emailjdlee@post.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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