L. monocytogenes FBUNT is able to form biofilm after six days at 10 ºC. Proteome under planktonic growth, lactocin AL705-treated and untreated biofilms was analyzed. Compared to planktonic cells, protein abundance shifts associated with ribosomes function and biogenesis, cell membrane functionality, carbohydrate and amino acid metabolism and transport were observed in sessile cells. When sessile cells were treated with lactocin AL705, proteins up-regulation were mostly related to carbohydrate metabolism and nutrient transport in an attempt to compensate for impaired energy generation caused by bacteriocin interacting with the cytoplasmic membrane. Notably, transport systems as β-glucosidase IIABC (lmo0027), cellobiose (lmo2763) and trehalose (lmo1255) specific PTS proteins were highly overexpressed.