Update publication information. Using bioinformatics analysis, we found that VP0057 may be a potential Ser/Thr protein kinase with phosphorylation activity. Thus, we constructed the vp0057-deletion mutant (∆vp0057) from the wild-type V. parahaemolyticus serotype O3:K6 and employed a mass spectrometry-based proteomic strategy to characterize proteome-wide changes in response to vp0057 deletion. One hundred ninety-seven differentially expressed proteins were identified in the ∆vp0057 strain compared with the wild-type strain, among which 135 proteins were upregulated and 62 proteins were downregulated.