PXD021706

PXD021706 is an original dataset announced via ProteomeXchange.

Title	Increased wheat protein content via introgression of a HMW glutenin selectively reshapes the grain proteome
Description	Introgression of a high molecular weight glutenin subunit (HMW-GS) gene, 1Ay21*, into commercial wheat cultivars increased overall grain protein content and bread-making quality by unknown mechanisms. As well as increased abundance of 1Ay HMW-GS, 115 differentially expressed proteins (DEPs) were discovered between three cultivars and corresponding introgressed near-isogenic lines (NILs). Functional category analysis showed that the DEPs were predominantly other storage proteins, and proteins involved in protein synthesis, protein folding, protein degradation, stress response and grain development. Nearly half the genes encoding the DEPs showed strong co-expression patterns during grain development. Promoters of these genes are enriched in elements associated with transcription initiation and light response, indicating a potential connection between these cis-elements and grain protein accumulation. A model of how this HMW-GS enhances the abundance of machinery for protein synthesis and maturation during grain filling is proposed. This analysis not only provides insights into how introgression of the 1Ay21* improves grain protein content, but also directs selection of protein candidates for future wheat quality breeding programmes.
HostingRepository	PRIDE
AnnounceDate	2021-05-30
AnnouncementXML	Submission_2021-05-30_22:54:01.567.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HUI CAO
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	No PTMs are included in the dataset
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-28 05:07:32	ID requested
1	2021-05-29 19:49:04	announced
⏵ 2	2021-05-30 22:54:02	announced	2021-05-31: Updated publication reference for PubMed record(s): 34000434.

Cao H, Duncan O, Islam S, Zhang J, Ma W, Millar AH, Increased Wheat Protein Content via Introgression of an HMW Glutenin Selectively Reshapes the Grain Proteome. Mol Cell Proteomics, 20():100097(2021) [pubmed]

submitter keyword: Wheat, mature grain, LC-MSMS

A.Harvey Millar
contact affiliation	ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Bayliss Building M316, Crawley, WA 6009, Australia
contact email	harvey.millar@uwa.edu.au
lab head
HUI CAO
contact affiliation	The University of Western Australia
contact email	hui.cao@uwa.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD021706

PRIDE project URI

• PRIDE
  1. PXD021706
     1. Label: PRIDE project
     2. Name: Increased wheat protein content via introgression of a HMW glutenin selectively reshapes the grain proteome