PXD021574

PXD021574 is an original dataset announced via ProteomeXchange.

Title	Cysteine oxidation and disulfide formation in the ribosomal exit tunnel
Description	Understanding the conformational sampling of translation-arrested ribosome nascent chain complexes is key to understand co-translational folding. Up to now, coupling of cysteine oxidation, disulfide bond formation and structure formation in nascent chains has remained elusive. Here, we investigate the eye-lens protein γB-crystallin in the ribosomal exit tunnel. Using mass spectrometry, theoretical simulations, dynamic nuclear polarization-enhanced solid-state nuclear magnetic resonance and cryo-electron microscopy, we show that thiol groups of cysteine residues undergo S-glutathionylation and S-nitrosylation and form non-native disulfide bonds. Thus, covalent modification chemistry occurs already prior to nascent chain release as the ribosome exit tunnel provides sufficient space even for disulfide bond formation which can guide protein folding.
HostingRepository	PRIDE
AnnounceDate	2020-11-09
AnnouncementXML	Submission_2020-11-09_04:48:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; nitrosylation; iodoacetamide derivatized residue; L-cysteine glutathione disulfide
Instrument	Orbitrap Fusion Lumos; impact II

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-20 23:38:16	ID requested
⏵ 1	2020-11-09 04:48:24	announced

Schulte L, Mao J, Reitz J, Sreeramulu S, Kudlinzki D, Hodirnau VV, Meier-Credo J, Saxena K, Buhr F, Langer JD, Blackledge M, Frangakis AS, Glaubitz C, Schwalbe H, Cysteine oxidation and disulfide formation in the ribosomal exit tunnel. Nat Commun, 11(1):5569(2020) [pubmed]

submitter keyword: Disulfide formation, ribosomal exit tunnel, cysterine oxidation, glutathione

Julian Langer
contact affiliation	MPI of Biophysics MPI for Brain Research Max von Laue Strasse 3/4 60438 Frankfurt am Main
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD021574

PRIDE project URI

• PRIDE
  1. PXD021574
     1. Label: PRIDE project
     2. Name: Cysteine oxidation and disulfide formation in the ribosomal exit tunnel