The proteomic makeup of three biological replicates of cells incubated for 24 h in MHB (control) or three biological replicates of cells incubated for 24 h in MHB containing 100 x MIC of penicillin G was analysed. The control or penicillin-treated cultures were stained with RSG and sorted using FACS into cells that did not fluoresce, cells that fluoresced weakly (dim) or cells that fluoresced brightly. Here we present the raw mass spectrometric data of control dimly stained cells and penicillin-treated brightly stained cells.