PXD021230-1

PXD021230 is an original dataset announced via ProteomeXchange.

Title	Single-Cell Transcriptomics and Phosphoproteomics Analyses Reveal Distinct Innate Immune Cell Polarization by Fibrin and Complement
Description	Innate immune cells are capable of polarizing to a range of effector functions including oxidative stress, antigen presentation, phagocytosis and cytokine secretion. However, how innate immune cells respond to extrinsic signals and whether unique polarization signatures govern their effector functions remains poorly understood. Here we report the ligand-selective transcriptome and phosphoproteome of innate immune cells by single-cell RNA-seq (scRNA-seq) and quantitative mass spectrometry. Innate immune cells exhibit unique polarization profiles upon ligand-selective activation induced by extrinsic molecular signals fibrin, complement, and LPS. While complement and LPS induced host defense and classical inflammatory states, respectively, fibrin induced prooxidant innate immune activation similar to the oxidative stress signature in neuroinflammatory disease. Thus, ligand-selective activation of innate immunity may be a key contributor of niche-specific polarization with implications for selective therapeutic targeting of pathogenic innate immune cells. This comprehensive dataset provides a resource for further investigation of innate immune cell functions in health and disease.
HostingRepository	PRIDE
AnnounceDate	2023-06-02
AnnouncementXML	Submission_2023-06-02_12:16:26.274.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JeffreyJohnson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-01 00:27:41	ID requested
⏵ 1	2023-06-02 12:16:26	announced
2	2023-06-09 05:21:44	announced	2023-06-09: Updated project metadata.
3	2023-11-14 09:00:56	announced	2023-11-14: Updated project metadata.

submitter keyword: neuroinflammation, itaconate, complement,Fibrinogen, macrophages, ROS, oxidative stress, microglia, transcriptomics

KaterinaAkassoglou
contact affiliation	Gladstone Institutes
contact email	katerina.akassoglou@gladstone.ucsf.edu
lab head
JeffreyJohnson
contact affiliation	Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021230
     1. Label: PRIDE project
     2. Name: Single-Cell Transcriptomics and Phosphoproteomics Analyses Reveal Distinct Innate Immune Cell Polarization by Fibrin and Complement