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PXD021149 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMS quantification of circulating CFP-10, p24 and VP40
DescriptionLiquid chromatography coupled mass spectrometry (LC-MS) operating in targeted mass spectrometry (MS) including multiple reaction monitoring (MRM) mode is an accurate analytical method. Integrating with nanoelectrospray-tandem MS (NanoES-MS/MS) and immunoprecipitation (IP), it becomes a promising method in detecting serum biomarkers which are at extremely low concentrations, such as mycobacterium tuberculosis (Mtb) secreted 10 kDa culture filtrate antigen (CFP-10). We employed an optimum denaturing condition for trypsin digestion and IP of targeted peptides. The condition shows several advantages over commonly used one, 1) it prevents further dilution of sample, thus enables as-needed sample volume, 2) It ensures liberation of trace amount of antigen peptide under a cost-effective enzyme to substrate mass ratio, 3) It avoids time-consuming and unnecessary reduction of disulfide bonds, as some pathogen antigens don't contain disulfide bond. More importantly, optimum MRM transitions of the targeted peptide and standardized data processing and interpretation algorithm provide confirmative information of it in serum samples. By changing the denaturing buffer, addition of a clean-up step, selection of optimum MRM transitions and better interpreting MS signal, this workflow showed an improved accuracy in clinical samples.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterQingbo Shu
SpeciesList scientific name: Human immunodeficiency virus 1; NCBI TaxID: 11676; scientific name: Sudan ebolavirus; NCBI TaxID: 186540; scientific name: Ebola virus; NCBI TaxID: 1570291; scientific name: Mycobacterium tuberculosis; NCBI TaxID: 1773;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentTSQ Altis; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-26 15:38:40ID requested
12021-10-19 10:24:04announced
22021-11-09 11:48:00announced: Added PubMed ID
Publication List
Shu Q, Kenny T, Fan J, Lyon CJ, Cazares LH, Hu TY, Species-specific quantification of circulating ebolavirus burden using VP40-derived peptide variants. PLoS Pathog, 17(11):e1010039(2021) [pubmed]
Keyword List
submitter keyword: MRM
Contact List
Tony Hu
contact affiliationTulane University
contact emailtonyhu@tulane.edu
lab head
Qingbo Shu
contact affiliationTulane University
contact emailqshu@tulane.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI