PXD020914

PXD020914 is an original dataset announced via ProteomeXchange.

Title	Functional analysis of the replication fork proteome identifies BET proteins as PCNA regulators
Description	Identifying proteins that function at replication forks is essential to understand DNA replication, chromatin assembly, and replication-coupled DNA repair mechanisms. Combining quantitative mass spectrometry in multiple cell types with stringent statistical cutoffs, we generated a high confidence catalogue of 593 proteins that are enriched at replication forks and nascent chromatin. Loss of function genetic analyses indicate that 85% yield phenotypes consistent with activities in DNA and chromatin replication or already have described functions in these processes. We illustrate the value of this resource by identifying activities of the BET family proteins BRD2, BRD3, and BRD4 in controlling DNA replication. These proteins use their extra-terminal domains to bind and inhibit the ATAD5 complex and thereby control the amount of PCNA on chromatin.
HostingRepository	PRIDE
AnnounceDate	2020-08-17
AnnouncementXML	Submission_2020-08-16_22:18:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Wessel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	isotope labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-14 08:21:19	ID requested
⏵ 1	2020-08-16 22:18:03	announced

Wessel SR, Mohni KN, Luzwick JW, Dungrawala H, Cortez D, Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep, 28(13):3497-3509.e4(2019) [pubmed]

submitter keyword: Replication, Replisome, iPOND, chromatin

David Cortez
contact affiliation	Department of Biochemistry, Vanderbilt University, USA
contact email	david.cortez@vanderbilt.edu
lab head
Sarah Wessel
contact affiliation	Vanderbilt University
contact email	sarah.r.wessel@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020914
     1. Label: PRIDE project
     2. Name: Functional analysis of the replication fork proteome identifies BET proteins as PCNA regulators