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PXD020748 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLabel-free quantification (LFQ) analysis of the AtACINUS interactome
DescriptionAtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modifications on AtACINUS. A homozygous 35S::AtACINUS-GFP/acinus-2 plant was selected for similar protein expression level to the endogenous AtACINUS protein of wild-type plants using a native α-AtACINUS antibody. We isolated putative AtACINUS interaction partners from young Arabidopsis seedlings using a modified LaG16LaG2 nanobody. Plants expressing TAP-GFP under 35S promoter were used as controls.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShouling Xu
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-05 09:59:52ID requested
12020-12-01 23:18:34announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plant, seedling, Arabidopsis thaliana, Label-free quantification analysis, LFQ, AtACINUS, ACINUS, co-immunoprecipitation, LC-MS/MS, IP-MS
Contact List
contact affiliationCarnegie Mass Spectrometry Facility
contact emailsxu@ciw.edu
lab head
Shouling Xu
contact affiliationCarnegie Institution at Stanford
contact emailsxu@ciw.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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