Updated publication reference for PubMed record(s): 32873710. Updated publication reference for DOI(s): 10.1074/jbc.RA120.015277. Actin was acetylated on lysine residues with acetic anhydride to determine its impaction on the contractile properties of the thin filaments including, actin's ability to undergo crossbridge cycling with myosin, actin's affinity for tropomyosin, and the impact of actin acetylation on calcium regulated thin filament activation