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PXD020516 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome variation with collagen yield in ancient bone
DescriptionIsotope analyses are some of the most common analytical methods applied to ancient bone, aiding the interpretation of past diets and chronology. For this, the evaluation of “collagen” yield is a routine step that allows for the selection of the specimens that are adequate for subsequent analyses, and samples with below ~1% yield normally discarded. Additionally, during the “collagen extraction” procedure, several sample fractions are generated and subsequently discarded but could contain proteins of analytical interest. In this study we evaluated the proteome variability of different fractions generated during the “collagen extraction” process of 29 samples, and their correlation with the collagen yield. We found that these fractions contained a significant amount of both collagenous and non-collagenous protein (NCP) spectra and that these were not correlated with the collagen yield. The variety of the extracted NCPs was comparable with that normally obtained from ancient samples, and the information obtained can be used to conduct species identification, phylogenetic studies, isotopic analyses and may be used to perform radiocarbon dating on the specimens. Overall, these findings suggest that not only is there value in retaining the fractions typically discarded as waste, but that low collagen yield specimens can still yield useful information.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterNoemi Procopio
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Cervus elaphus; NCBI TaxID: 9860;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-22 22:50:42ID requested
12021-02-07 23:31:16announced
Publication List
Procopio N, Hopkins RJA, Harvey VL, Buckley M, Proteome Variation with Collagen Yield in Ancient Bone. J Proteome Res, 20(3):1754-1769(2021) [pubmed]
Keyword List
submitter keyword: Proteomics
ancient bone
radiocarbon dating
stable isotopes.
Contact List
Michael Buckley
contact affiliationManchester Institute of Biotechnology, 131 Princess Street, The University of Manchester, Manchester, M1 7DN, UK
contact emailm.buckley@manchester.ac.uk
lab head
Noemi Procopio
contact affiliationNorthumbria University
contact emailnoemi.procopio@northumbria.ac.uk
dataset submitter
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Dataset FTP location
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