Grain samples of Hordeum vulgare L. sensu lato variety Sebastian were obtained from plants grown in the fields at four breeding stations in 2017, representing seed development under different combinations of temperature and rainfall. Sample description: CAS [1-4] - location n. 5 - (Caslav) - high yield, above average temperature and high rainfall. UHO [1-4] - location n. 11 (Uhersky Ostroh) - low yield, high temperature and low rainfall. CHA [1-4] - location n. 2 (Chrastava) - average yield, low temperature, very high rainfall. KRU [1-4] - location n. 8 (Krasne Udoli) - average yield, low temperature, average rainfall.