PXD020022

PXD020022 is an original dataset announced via ProteomeXchange.

Title	Recombinant Tau and EFhd2 from Bacteria
Description	E. coli is a commonly used host for generating large quantities of human recombinant proteins. These proteins are often considered free of post-translational modifications (PTMs); however, a subset of the produced proteins can incorporate modifications derived from the host bacterial enzymes. Here, we used tandem mass spectrometry to identify inadvertent PTMs on human recombinant proteins produced in E. coli. As proof of concept, we identified bacteria-mediated PTMs on human tau and EFhd2 proteins, which we used in in vitro studies to understand their role in Alzheimer's disease (AD). Some of the identified PTMs have been previously associated with AD while others are novel. Additionally, we identified E. coli proteins that co-purified with these human recombinant proteins even after several purification steps.
HostingRepository	PRIDE
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-08_08:19:09.175.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020022
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jared Lamp
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-25 18:18:16	ID requested
⏵ 1	2025-07-08 08:19:09	announced

Umstead A, Soliman AS, Lamp J, Vega IE, Validation of recombinant human protein purified from bacteria: An important step to increase scientific rigor. Anal Biochem, 611():113999(2020) [pubmed]

10.6019/PXD020022;

10.1016/j.ab.2020.113999;

submitter keyword: E. Coli, Tau,PTM, Recombinant, EFhd2, LC-MS/MS

Irving Vega
contact affiliation	Integrated Mass Spectrometry Unit, Michigan State University, Grand Rapids Research Center, USA
contact email	vegaie@msu.edu
lab head
Jared Lamp
contact affiliation	Michigan State University
contact email	lampjare@msu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD020022

PRIDE project URI

• PRIDE
  1. PXD020022
     1. Label: PRIDE project
     2. Name: Recombinant Tau and EFhd2 from Bacteria