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PXD019846 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReciprocal growth control by competitive binding of nucleotide second messengers to a metabolic switch in Caulobacter crescentus
DescriptionBacteria use a range of small signaling molecules to tune their physiology in response to changes in the environment. It has remained unclear if these regulatory networks operate independently or if they interact to optimize bacterial growth and survival. Here, we demonstrate that (p)ppGpp and c-di-GMP reciprocally regulate growth of Caulobacter crescentus by converging on a single small-molecule-binding protein, SmbA. Both second messengers bind to SmbA with high affinity and in a competitive manner, with the guanine moiety of (p)ppGpp and one of the guanyl bases of dimeric c-di-GMP forming identical interactions with SmbA. While c-di-GMP binding inhibits SmbA, (p)ppGpp interferes with this inhibition to sustain SmbA activity. We demonstrate that (p)ppGpp specifically promotes Caulobacter growth on glucose, while c-di-GMP inhibits glucose consumption. We find that SmbA contributes to this metabolic switch and promotes growth on glucose by quenching redox stress under these conditions. The identification of the first effector protein that acts as a central regulatory hub for two global second messengers opens up future studies on specific cross-talk between small-molecule-based regulatory networks.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterThomas Bock
SpeciesList scientific name: Caulobacter vibrioides NA1000; NCBI TaxID: 565050;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-17 15:40:48ID requested
12020-12-07 22:43:25announced
Publication List
Shyp V, Dubey BN, B, รถ, hm R, Hartl J, Nesper J, Vorholt JA, Hiller S, Schirmer T, Jenal U, Reciprocal growth control by competitive binding of nucleotide second messengers to a metabolic switch in Caulobacter crescentus. Nat Microbiol, 6(1):59-72(2021) [pubmed]
Keyword List
submitter keyword: ppGpp, c-di-GMP, glucose metabolism, adaptation
Contact List
Alexander Schmidt
contact affiliationProteomics Core Facility Biozentrum, University of Basel Klingelbergstrasse 50/70 CH-4056 Basel / Switzerland
contact emailalex.schmidt@unibas.ch
lab head
Thomas Bock
contact affiliationProteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact emailthomas.bock@unibas.ch
dataset submitter
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Dataset FTP location
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