PXD019795 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit |
Description | APC/C-mediated proteolysis of cyclin B and securin promotes entry into anaphase, inactivating CDK1 and permitting chromosome segregation, respectively. Reduction of CDK1 activity relieves inhibition of the CDK1-opposing phosphatases PP1 and PP2A-B55 leading to dephosphorylation of substrates crucial for mitotic exit. Meanwhile, continued APC/C activity is required to target various proteins, including Aurora and Polo kinases, for degradation. Together, these activities orchestrate a complex series of events during mitotic exit. However, the relative importance of regulated proteolysis and dephosphorylation in dictating the order and timing of these events remains unclear. Using high temporal-resolution mass spectrometry, we compare the relative extent of proteolysis and protein dephosphorylation. This reveals highly-selective rapid (~5min half-life) proteolysis of cyclin B, securin and geminin at the metaphase to anaphase transition, followed by slow proteolysis (>60 min half-life) of other mitotic regulators. Protein dephosphorylation requires APC/C-dependent destruction of cyclin B and was resolved into PP1-dependent fast, intermediate and slow categories with unique sequence motifs. We conclude that dephosphorylation initiated by the selective proteolysis of cyclin B drives the bulk of changes observed during mitotic exit. |
HostingRepository | PRIDE |
AnnounceDate | 2020-09-04 |
AnnouncementXML | Submission_2020-09-04_04:28:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Holder |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-15 16:41:48 | ID requested | |
⏵ 1 | 2020-09-04 04:28:27 | announced | |
2 | 2023-11-14 08:51:09 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Mitosis, mitotic exit, proteolysis, proteome, APC/C, cell cycle |
Contact List
Francis Barr |
contact affiliation | Department of Biochemistry, University of Oxford |
contact email | francis.barr@bioch.ox.ac.uk |
lab head | |
James Holder |
contact affiliation | University of Oxford |
contact email | james.holder@path.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019795
- Label: PRIDE project
- Name: Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit