Updated project metadata. In this study, we scrutinized by comparative proteomics using Tandem Mass Tags (TMT) couple to synchronous-precursor selection (SPS)-MS3 the dynamic of the proteome of specific tissues of avocado seeds (testa, cotyledon, and embryo) during postharvest shelf life. Our proteomics pipeline allows the identification of more proteins in testa compared to other tissues, and also, we could track the dynamic of more protein in this tissue during PSL. In general, proteins associated with stress, glycolic process, and starch metabolism were commonly identify in each tissue. In contracts, a drastic difference in protein translation, amino acid, and fatty acid metabolism was observed among tissues during PSL.