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<ProteomeXchangeDataset xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" id="PXD019160" xsi:noNamespaceSchemaLocation="proteomeXchange-1.4.0.xsd" formatVersion="1.4.0">
  <CvList>
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    <Cv id="UNIMOD" fullName="UNIMOD" uri="http://www.unimod.org/obo/unimod.obo"/>
  </CvList>
  <ChangeLog>
    <ChangeLogEntry date="2022-09-18">Updated FTP location.</ChangeLogEntry>
  </ChangeLog>
  <DatasetSummary announceDate="2020-05-13" hostingRepository="jPOST" title="Nucleosome binding by the pioneer transcription factor OCT4">
    <Description>The nucleosome is a fundamental unit of chromatin in eukaryotes, and generally prevents the binding of transcription factors to genomic DNA. Pioneer transcription factors overcome the nucleosome barrier, and bind their target DNA sequences in chromatin. OCT4 is a representative pioneer transcription factor that plays a role in stem cell pluripotency. In the present study, we biochemically analyzed the nucleosome binding by OCT4. Crosslinking mass spectrometry showed that OCT4 binds the nucleosome.</Description>
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      <cvParam cvRef="MS" accession="MS:1002854" name="Peer-reviewed dataset"/>
    </ReviewLevel>
    <RepositorySupport>
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  </DatasetSummary>
  <DatasetIdentifierList>
    <DatasetIdentifier>
      <cvParam accession="MS:1001919" cvRef="MS" name="ProteomeXchange accession number" value="PXD019160"/>
      <cvParam accession="MS:1001921" cvRef="MS" name="ProteomeXchange accession number version number" value="4"/>
    </DatasetIdentifier>
    <DatasetIdentifier>
      <cvParam accession="MS:1002632" cvRef="MS" name="jPOST dataset identifier" value="JPST000844"/>
    </DatasetIdentifier>
  </DatasetIdentifierList>
  <DatasetOriginList>
    <DatasetOrigin>
      <cvParam cvRef="MS" accession="MS:1002868" name="Original data"/>
    </DatasetOrigin>
  </DatasetOriginList>
  <SpeciesList>
    <Species>
      <cvParam cvRef="MS" accession="MS:1001469" name="taxonomy: scientific name" value="Homo sapiens (Human)"/>
      <cvParam cvRef="MS" accession="MS:1001467" name="taxonomy: NCBI TaxID" value="9606"/>
    </Species>
  </SpeciesList>
  <InstrumentList>
    <Instrument id="Instrument_1">
      <cvParam cvRef="MS" accession="MS:1001742" name="LTQ Orbitrap Velos"/>
    </Instrument>
  </InstrumentList>
  <ModificationList>
    <cvParam cvRef="MOD" accession="MOD:01060" name="S-carboxamidomethyl-L-cysteine"/>
    <cvParam cvRef="MOD" accession="MOD:00719" name="L-methionine sulfoxide"/>
    <cvParam cvRef="MS" accession="MS:1001460" name="unknown modification"/>
  </ModificationList>
  <ContactList>
    <Contact id="project_submitter">
      <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Lumi Negishi"/>
      <cvParam cvRef="MS" accession="MS:1000590" name="contact affiliation" value="the University of Tokyo"/>
      <cvParam cvRef="MS" accession="MS:1002037" name="dataset submitter"/>
    </Contact>
    <Contact id="project_lab_head">
      <cvParam cvRef="MS" accession="MS:1002332" name="lab head"/>
      <cvParam cvRef="MS" accession="MS:1000586" name="contact name" value="Kurumizaka Hitoshi"/>
    </Contact>
  </ContactList>
  <PublicationList>
    <Publication id="PMID32678275">
      <cvParam cvRef="MS" accession="MS:1000879" name="PubMed identifier" value="32678275"/>
      <cvParam cvRef="MS" accession="MS:1002866" name="Reference" value="Echigoya K, Koyama M, Negishi L, Takizawa Y, Mizukami Y, Shimabayashi H, Kuroda A, Kurumizaka H Nucleosome binding by the pioneer transcription factor OCT4. Sci Rep. 2020 Jul 16;10(1):11832"/>
    </Publication>
  </PublicationList>
  <KeywordList>
    <cvParam cvRef="MS" accession="MS:1001925" name="submitter keyword" value="nucleosome, chromatin, OCT4, Crosslinking MS"/>
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      <cvParam accession="MS:1002633" cvRef="MS" name="jPOST dataset URI" value="https://repository.jpostdb.org/entry/JPST000844"/>
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    <FullDatasetLink>
      <cvParam cvRef="MS" accession="MS:1002852" name="Dataset FTP location" value="ftp://ftp.jpostdb.org/JPST000844/"/>
    </FullDatasetLink>
  </FullDatasetLinkList>
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