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PXD019061
PXD019061 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of Immune Checkpoint Drug Targets and Tumor Proteotypes in Non-Small Cell Lung Cancer |
| Description | New therapeutics targeting immune checkpoint proteins have significantly advanced treatment of non-small cell lung cancer (NSCLC), but protein level quantitation of drug targets presents a critical problem. We used multiplexed, targeted mass spectrometry (MS) to quantify the immunotherapy target proteins PD-1, PD-L1, PD-L2, IDO1, LAG3, TIM-3, VISTA, GITR, and CD40 in formalin-fixed, paraffin-embedded (FFPE) NSCLC specimens. Immunohistochemistry (IHC) and MS measurements for PD-L1 were weakly correlated, but IHC did not distinguish protein abundance differences detected by MS. PD-L2 abundance exceeded PD-L1 in over half the specimens and the drug target proteins all displayed different abundance patterns. mRNA correlated with protein abundance only for PD-1, PD-L1, and IDO1 and tumor mutation burden did not predict abundance of any protein targets. Global proteome analyses identified distinct proteotypes associated with high PD-L1-expressing and high IDO1-expressing NSCLC. MS quantification of multiple drug targets and tissue proteotypes can improve clinical evaluation of immunotherapies for NSCLC. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2020-06-22 |
| AnnouncementXML | Submission_2020-06-22_05:42:47.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ryan Morrison |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4) |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-05-08 15:45:17 | ID requested | |
| ⏵ 1 | 2020-06-22 05:42:48 | announced |
Publication List
Keyword List
| submitter keyword: FFPE NSCLC PRM |
Contact List
| Daniel Liebler | |
|---|---|
| contact affiliation | Protypia, Inc |
| contact email | daniel.liebler@protypia.com |
| lab head | |
| Ryan Morrison | |
| contact affiliation | Protypia, Inc |
| contact email | ryan.morrison@protypia.com |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
