Trametenolic acid B (TAB) is a triterpenoid extracted from the Laetiporus sulphureus (Fr.) Murrill, which can effectively inhibit the proliferation of HepG2/2.215 cells and induce the autophagy. Proteomic analysis was performed to further study the anti-cancer mechanism of TAB. According to the peptide segment quantitative standard (FDR≤1%)，a total of 5324 proteins were identified in HepG2/2.215 by proteomic analysis.