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PXD018762 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated multi-omics reveals common properties underlying stress granule and P-body formation
DescriptionNon-membrane-bound compartments such as mRNA processing bodies (PBs) and stress granules (SGs) play important roles in the regulation of gene expression following environmental stresses. We have systematically determined the protein and mRNA composition of PBs and SGs formed in response to a common stress condition imposed by glucose depletion. We find that high molecular weight (HMW) complexes exist prior to glucose depletion that may act as seeds for the further condensation of proteins forming mature PBs and SGs. Both before and after glucose depletion, these HMW complexes are enriched for proteins containing low complexity and RNA binding domains. The mRNA content of these HMW complexes is enriched for long, structured mRNAs that become more poorly translated following glucose depletion. Many proteins and mRNAs are shared between PBs and SGs, suggesting a complex pattern of functional protein and mRNA pools exist in different condensates. Even where the precise identity of mRNAs and proteins localizing to PBs and SGs is distinct, the mRNAs and proteins share common biophysical and chemical features that likely trigger their phase separation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Nelson
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-23 05:27:47ID requested
12021-11-03 08:02:25announced
Publication List
Kershaw CJ, Nelson MG, Lui J, Bates CP, Jennings MD, Hubbard SJ, Ashe MP, Grant CM, Integrated multi-omics reveals common properties underlying stress granule and P-body formation. RNA Biol, 18(sup2):655-673(2021) [pubmed]
Keyword List
submitter keyword: Saccharomyces cerevisiae, P-body purification, Stress Granule purification
Contact List
Professor Simon Hubbard
contact affiliationDivision of Evolution and Genomic Sciences School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Oxford Road, Manchester, M13 9PT
contact emailsimon.hubbard@manchester.ac.uk
lab head
Michael Nelson
contact affiliationThe University of Manchester
contact email42mnelson@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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