PXD018749 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation |
Description | In eukaryotes, trimethylation of lysine 9 on histone H3 (H3K9) is associated with transcriptional silencing of transposable elements (TEs). In drosophila ovaries, this heterochromatic repressive mark is thought to be deposited by SetDB1 on TE genomic loci after the initial recognition of nascent transcripts by PIWI-interacting RNAs (piRNAs) loaded on the Piwi protein. Here, we show that the nucleosome remodeler Mi-2, in complex with its partner MEP-1, forms a subunit that is transiently associated, in a MEP-1 C-terminus-dependent manner, with known Piwi interactors, including a recently reported SUMO ligase Su(var)2-10. Together with the histone deacetylase Rpd3, this module is involved in the piRNA-dependent TE silencing, correlated with H3K9 deacetylation and trimethylation. Therefore. drosophila piRNA-mediated transcriptional silencing involves three epigenetic effectors, a remodeler, Mi-2, an eraser, Rpd3 and a writer, SetDB1, in addition to the Su(var)2-10 SUMO ligase. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:09:29.599.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018749 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | severine chambeyron |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | Methyl; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-23 00:47:28 | ID requested | |
1 | 2020-06-02 18:31:02 | announced | |
2 | 2020-06-09 23:30:16 | announced | 2020-06-10: Updated publication reference for PubMed record(s): 32499524. |
⏵ 3 | 2024-10-22 05:09:33 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.6019/PXD018749; |
Mugat B, Nicot S, Varela-Chavez C, Jourdan C, Sato K, Basyuk E, Juge F, Siomi MC, P, é, lisson A, Chambeyron S, The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation. Nat Commun, 11(1):2818(2020) [pubmed] |
10.1038/s41467-020-16635-5; |
Keyword List
submitter keyword: MEP-1, RPD3, Mi-2,Gtsf1 |
Contact List
CHAMBEYRON SEVERINE |
contact affiliation | Institut Of Human Genetics, CNRS, Univ. Montpellier, UMR 9002, France |
contact email | Severine.chambeyron@igh.cnrs.fr |
lab head | |
severine chambeyron |
contact affiliation | CNRS-Université de Montepllier |
contact email | severine.chambeyron@igh.cnrs.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD018749 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018749
- Label: PRIDE project
- Name: The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation