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PXD018563 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMitochondrial dysfunction caused by bacterial outer membranes vesicles activates intrinsic apoptosis and inflammation
DescriptionSensing of microbes activates the immune system, depending on functional mitochondria. However, pathogenic bacteria inhibit mitochondria activity by delivering toxins via outer membrane vesicles (OMVs). How innate immune cells respond to pathogenic microbes that target mitochondria remains unclear. Here, we show that macrophages induce mitochondrial apoptosis and the NLRP3 inflammasome in response OMVs. Macrophages treated with OMVs and toxins that cause mitochondria dysfunction cease host protein translation which depletes pro-survival BCL-2 family member, MCL-1, and induces BAK-dependent mitochondrial apoptosis. Mitochondrial apoptosis and potassium ion efflux activate the NLRP3 inflammasome after OMV exposure. Importantly, mitochondrial apoptosis modulates IL-1 serum levels in response to OMVs. Our data highlight how innate immune cells sense infections by monitoring mitochondrial health.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCheng Huang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-15 00:50:31ID requested
12020-07-30 00:54:32announced
22020-08-22 16:44:28announced2020-08-23: Updated publication reference for PubMed record(s): 32807891.
Publication List
Deo P, Chow SH, Han ML, Speir M, Huang C, Schittenhelm RB, Dhital S, Emery J, Li J, Kile BT, Vince JE, Lawlor KE, Naderer T, Mitochondrial dysfunction caused by outer membrane vesicles from Gram-negative bacteria activates intrinsic apoptosis and inflammation. Nat Microbiol, 5(11):1418-1427(2020) [pubmed]
Keyword List
submitter keyword: macrophage, mitochondria, cell death, pyroptosis, apoptosis, toxin, OMV, inflammation, Neisseria, bacteria
Contact List
Thomas Naderer
contact affiliationDepartment of Biochemistry & Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton 3800, Australia
contact emailthomas.naderer@monash.edu
lab head
Cheng Huang
contact affiliationMonash University
contact emailcheng.huang@monash.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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