Updated project metadata. In this study we developed a stable isotope labeled of mammals (SILAM) model coupled to the application of differential systemic decellularization in vivo (DISDIVO) to evaluate the molecular dynamics of whole organism vascular beds at the initial phase of acute sepsis. Three different vascular layers where characterized at the initial stage of sepsis, glycocalyx (GC), endothelial cells (ECs) and smooth muscle cells (SMCs).