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PXD017729 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDrosophila SWR1 and NuA4 complexes are defined by DOMINO isoform
DescriptionHistone acetylation and deposition of H2A.Z variant are integral aspects of active transcrip-tion. In Drosophila, the single DOMINO chromatin regulator complex is thought to combine both activities via an unknown mechanism. Here we show that alternative isoforms of the DOMINO nucleosome remodeling ATPase, DOM-A and DOM-B, directly specify two distinct multi-subunit complexes. Both complexes are necessary for transcriptional regulation but through different mechanisms. The DOM-B complex incorporates H2A.V (the fly ortholog of H2A.Z) genome-wide in an ATP-dependent manner, like the yeast SWR1 complex. The DOM-A complex, instead, functions as an ATP-independent histone acetyltransferase com-plex similar to the yeast NuA4, targeting lysine 12 of histone H4. Our work provides an in-structive example of how different evolutionary strategies lead to similar functional separation. In yeast and humans, nucleosome remodeling and histone acetyltransferase complexes orig-inate from gene duplication and paralog specification. Drosophila generates the same diversi-ty by alternative splicing of a single gene.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlessandro Scacchetti
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListacetylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-26 00:16:16ID requested
12020-05-26 16:13:05announced
Publication List
Scacchetti A, Schauer T, Reim A, Apostolou Z, Campos Sparr A, Krause S, Heun P, Wierer M, Becker PB, SWR1 and NuA4 complexes are defined by DOMINO isoforms. Elife, 9():(2020) [pubmed]
Keyword List
submitter keyword: Drosophila, DOMINO, SWR1, NuA4, H3ac, H4ac
Contact List
Peter B. Becker
contact affiliationMolecular Biology Division, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany
contact emailpbecker@bmc.med.lmu.de
lab head
Alessandro Scacchetti
contact affiliationMolecular Biology Division, Biomedical Center, Ludwig-Maximilians-University, Munich
contact emailAlessandro.Scacchetti@med.uni-muenchen.de
dataset submitter
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Dataset FTP location
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