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PXD017703 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleParallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF)
DescriptionData independent acquisition modes isolate and concurrently fragment populations of different precursors by cycling through segments of a predefined precursor m/z range. Although these selection windows collectively cover the entire m/z range, overall only a few percent of all incoming ions in each window are sampled. Making use of the correlation of molecular weight and ion mobility in a trapped ion mobility device (timsTOF Pro), we here devise a novel scan mode that samples up to 100% of the peptide precursor ion current in m/z and mobility windows. We extend an established targeted data extraction workflow by including the ion mobility dimension for both signal extraction and scoring, thereby increasing the specificity for precursor identification. Data acquired from whole proteome digests and mixed organism samples demonstrate deep proteome coverage and a very high degree of reproducibility as well as quantitative accuracy, even from 10 ng sample amounts.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-24 06:08:48ID requested
12020-09-22 02:08:40announced
22020-12-06 23:32:28announced2020-12-07: Updated publication reference for PubMed record(s): 33257825.
Publication List
Meier F, Brunner AD, Frank M, Ha A, Bludau I, Voytik E, Kaspar-Schoenefeld S, Lubeck M, Raether O, Bache N, Aebersold R, Collins BC, R, ö, st HL, Mann M, diaPASEF: parallel accumulation-serial fragmentation combined with data-independent acquisition. Nat Methods, 17(12):1229-1236(2020) [pubmed]
Keyword List
submitter keyword: technical, ion mobility, DIA, TIMS, PASEF
Contact List
Matthias Mann
contact affiliationProteomics and Signal Transduction Max Planck Institute of Biochemistry Germany
contact emailmmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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