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PXD017620 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCross-linking mass spectrometry of yeast mitochondria grown on different carbon sources reveal Ndi1, Min8 and Pet9 associated with the electron transport chain
DescriptionMitochondria derived from Saccharomyces cerevisiae grown on either a non-fermentable (glycerol) or a fermentable (glucose) carbon source were cross-linked with BS3. Additionally, by using a stable-isotope labelled quantitative cross-linking approach, we were able to quantify differences in protein-protein cross-links in mitochondria according to the growth condition. Furthermore, so far uncharacterized yeast proteins were put into biological context based on their cross-linking pattern.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Linden
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Orbitrap Fusion Lumos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-19 07:42:40ID requested
12020-04-27 00:27:12announced
22020-07-15 00:20:43announced2020-07-15: Updated publication reference for PubMed record(s): 32332106.
Publication List
Linden A, Deckers M, Parfentev I, Pflanz R, Homberg B, Neumann P, Ficner R, Rehling P, Urlaub H, A Cross-linking Mass Spectrometry Approach Defines Protein Interactions in Yeast Mitochondria. Mol Cell Proteomics, 19(7):1161-1178(2020) [pubmed]
Keyword List
submitter keyword: cross-linking, mass spectrometry, XL-MS, quantitative, qXL-MS, mitochondria, yeast, Saccharomyces cerevisiae, carbon source
Contact List
Henning Urlaub
contact affiliationMax Planck Institute for Biophysical Chemistry, Göttingen, Germany and Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany
contact emailHenning.Urlaub@mpibpc.mpg.de
lab head
Andreas Linden
contact affiliationMax Planck Institute for Biophysical Chemistry and Clinical Chemistry, University Medical Center Göttingen
contact emailAndreas.Linden@mpibpc.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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