Chinese wheat mosaic virus (CWMV) is a severe threat to winter wheat, but the mechanism of the viral pathogenic mechanism is poorly understood. In this study, a LC-MS/MS-based quantitative proteomics was conducted to analyze the proteomic changes of CWMV-infected Nicotiana benthamiana compared to mock plants. In total, 2,751 identified host proteins were identified, 1,496 of which were quantified. 146 up-regulated and 248 down-regulated proteins were characterized as differently accumulated proteins (DAPs)