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PXD017423 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBis(zinc(II)-dipicolylamine) functionalized sub-2 μm core-shell microspheres enabled comprehensive analysis of N-phosphoproteome
DescriptionHerein, bis(zinc(II)-dipicolylamine) functionalized sub-2 μm core-shell silica microspheres (SiO2@DpaZn) were tailored for N-phosphopeptide enrichment at pH 7.7. Contributed by the coordination bonding between Zn(II) and phosphate groups with the KD value of 13.14 μM, N-phosphopeptides could be captured efficiently under neutral conditions. Moreover, contributed by the core-shell structure of SiO2@DpaZn, the fast on-tip enrichment ensured the recovery of N-phosphopeptides up to 44.1%, 3-fold higher than that obtained by in-solution capture. Moreover, 40 determined N-pho sites on 32 substrates were identified in E. coli lysates, among which five had kinase activity. All these results demonstrate that our method is beneficial to promote the discovery of N-phosphoproteins with significant biological functions.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYechen Hu
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListphosphorylated residue
InstrumentQ Exactive; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-02-06 23:26:45ID requested
12020-02-06 23:28:09announced
22023-03-01 19:24:20announced2023-03-02: Update publication information.
Publication List
Hu Y, Jiang B, Weng Y, Sui Z, Zhao B, Chen Y, Liu L, Wu Q, Liang Z, Zhang L, Zhang Y, m core-shell microspheres for the analysis of N-phosphoproteome. Nat Commun, 11(1):6226(2020) [pubmed]
Keyword List
submitter keyword: Bis(zinc(II)-dipicolylamine) , sub-2 μm core-shell microspheres, N--phosphoproteome
Contact List
Lihua Zhang
contact affiliationDalian Institute of Chemical physics, CAS
contact emaillihuazhang@dicp.ac.cn
lab head
Yechen Hu
contact affiliationDalian Institute of Chemical physics, CAS
contact emailhuyechen@njmu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI