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PXD017022 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTRF1 averts chromatin remodelling, recombination and replication dependent-Break Induced Replication at mouse telomeres
DescriptionTelomeric repeat sequences are prone to induce replication stress and one of the core telomere binding proteins, TRF1, is thought to counteract these problems. The molecular mechanisms coming into play upon TRF1 loss were not clearly defined though. This study combines an inducible TRF1 loss with telomere-targeted proteomics to analyze the incurring changes. The results show that loss of TRF1 causes important alterations in telomere associated proteins and the new profile resembles in a number of ways that of cells in which telomeres are maintained by telomerase-independent mechanisms, named ALT. The presence of DNA repair proteins (MRN complex, the SMC5/6 complex), some increases in telomere colocalization with PML bodies, sister chromatid exchanges and clear signs of increased TERRA transcription and mitotic break-induced replication are part of these phenotypes. The data also show that these early ALT phenotypes can be differentiated from drug induced replication stress, and part of this ALT response is conserved in human cells. These studies therefore point to a critical role for TRF1 in preventing the emergence of ALT and hence immortalization of cells. Given that the details for how ALT actually is initiated remained unclear, these findings are an important step forward and will spur new research into that question.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlex Montoya
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-09 00:20:27ID requested
12020-01-22 07:42:10announced
Publication List
Porreca RM, Herrera-Moyano E, Skourti E, Law PP, Gonzalez Franco R, Montoya A, Faull P, Kramer H, Vannier JB, TRF1 averts chromatin remodelling, recombination and replication dependent-break induced replication at mouse telomeres. Elife, 9():(2020) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Mus Musculus, Embryonic Fibroblasts, Teleomere, Shelterin complex, LC-MSMS
Contact List
Jean-Baptiste Vannier
contact affiliationMedical Research Council - London Institute of Medical Sciences, Telomere Replication and Stability Group, United Kingdom.
contact emailj.vannier@lms.mrc.ac.uk
lab head
Alex Montoya
contact affiliationMedical Research Council - London Institute of Medical Sciences
contact emailalex.montoya@lms.mrc.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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