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PXD016940 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTMTpro16plex Isobaric Labeling Reagents: Attaining New Sample Multiplexing Heights and New Cellular Proteome Depths in Quantitative Proteomics
DescriptionIsobaric labeling empowers proteome-wide expression measurements simultaneously across multiple samples. Here, an expanded set of 16 isobaric reagents based on a proline backbone (TMTpro) is presented. These reagents have similar characteristics to existing TMT reagents but with increased fragmentation efficiency and signal. In a proteome-scale example dataset, we compared eight common cell lines with and without Torin1 treatment with three replicates, quantifying more than 8,800 proteins (mean of 7.5 peptides/protein) per replicate with an analysis time of only 67 min per proteome examined. Finally, we modified the powerful thermal stability assay to examine proteome-wide melting shifts after treatment with DMSO, 1 or 20 ������M staurosporine with five replicates. This new assay identified and dose-stratified the staurosporine binding to 228 cellular kinases in just one, 18-hr experiment. TMTpro reagents allow unprecedented complexity in experimental designs���������all with basically no missing values across the 16 samples and no loss in quantitative integrity.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiaming Li
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-01-05 22:01:41ID requested
12020-03-19 16:35:38announced
22020-03-25 07:19:27announced2020-03-25: Updated publication reference for PubMed record(s): 32203386.
Publication List
Li J, Van Vranken JG, Pontano Vaites L, Schweppe DK, Huttlin EL, Etienne C, Nandhikonda P, Viner R, Robitaille AM, Thompson AH, Kuhn K, Pike I, Bomgarden RD, Rogers JC, Gygi SP, Paulo JA, TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples. Nat Methods, 17(4):399-404(2020) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Human, cell lines, SPS-MS3, TMTpro, proteomics
Contact List
Steven P. Gygi
contact affiliationDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA
contact emailSteven_Gygi@hms.harvard.edu
lab head
Jiaming Li
contact affiliationHarvard Medical School
contact emailjiaming_li@hms.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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