PXD016851 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Study of APOL1-containing complexes in podocytes |
Description | The C-terminal variants G1 and G2 of apolipoprotein L1 (APOL1) confer human resistance to the sleeping sickness parasite Trypanosoma rhodesiense, but also increase the risk of developing kidney disease. APOL1 and APOL3 are death-promoting proteins associated with endoplasmic reticulum and Golgi membranes. We report that in podocytes, either expression of APOL1 devoid of C-terminal helix (APOL1) or deletion of APOL3 (APOL3 KO) induce actomyosin reorganization linked to inhibition of phosphatidylinositol-4-phosphate (PI(4)P) synthesis by the Golgi PI(4)-kinase IIIB (PI4KB). Both APOL1 and APOL3 form K+ channels, but only APOL3 exhibited Ca2+-dependent binding to neuronal calcium sensor-1 (NCS-1), promoting NCS-1/PI4KB interaction that strongly activates PI(4)P synthesis. APOL1 C-terminal deletion or mutations increased APOL1 binding to APOL3, affecting APOL3 interaction with NCS-1. Since podocytes of G1 and G2 patients exhibited an APOL1/APOL3KO-like phenotype, APOL1 C-terminal variants may induce kidney disease by preventing APOL3 from activating PI4KB, resulting in actomyosin reorganization of podocytes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-03-19 |
AnnouncementXML | Submission_2020-03-19_06:54:30.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD016851 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marc Dieu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Ammonia-loss; 2-pyrrolidone-5-carboxylic acid (Gln); Dehydrated; Oxidation; Acetyl; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue; Gln->pyro-Glu |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-12-19 07:03:13 | ID requested | |
⏵ 1 | 2020-03-19 06:54:31 | announced | |
2 | 2020-06-12 02:59:39 | announced | 2020-06-12: Updated publication reference for PubMed record(s): 32187552. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: APOL1, Kidney disease, podocytes |
Contact List
Etienne PAYS |
contact affiliation | Laboratory of Molecular Parasitology, IBMM, Université Libre de Bruxelles, B-6041 Gosselies, Belgium |
contact email | etiennepays@gmail.com |
lab head | |
Marc Dieu |
contact affiliation | University of Namur - MaSUN |
contact email | marc.dieu@unamur.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016851
- Label: PRIDE project
- Name: Study of APOL1-containing complexes in podocytes