PXD016845

PXD016845 is an original dataset announced via ProteomeXchange.

Title	MS-based quantitative proteomic analysis of total proteomes from T. gondii strains upon MORC���s depletion and TgHDAC3 inhibition.
Description	T. gondii has a complex life cycle typified by an asexual development taking place in vertebrate, and a sexual reproduction which occurs exclusively in felids and thereby is less studied. The developmental transitions rely on changes in gene expression patterns, and recent studies have assigned roles for chromatin shapers, including histone modifications, in establishing specific epigenetic programs for each given stage. Here, we identified T. gondii microrchidia (MORC) protein as an upstream transcriptional repressor of sexual commitment. To explore the molecular role of MORC, we conducted mass spectrometry-based quantitative proteomic analyses to monitor the proteome changes upon MORC���s depletion and TgHDAC3 inhibition.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-04-14_03:13:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yohann Cout��
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-12-19 06:08:52	ID requested
1	2020-01-09 11:37:17	announced
⏵ 2	2020-04-14 03:13:37	announced	2020-04-14: Updated publication reference for PubMed record(s): 32094587.

Farhat DC, Swale C, Dard C, Cannella D, Ortet P, Barakat M, Sindikubwabo F, Belmudes L, De Bock PJ, Cout, é Y, Bougdour A, Hakimi MA, A MORC-driven transcriptional switch controls Toxoplasma developmental trajectories and sexual commitment. Nat Microbiol, 5(4):570-583(2020) [pubmed]

curator keyword: Biomedical

submitter keyword: Toxoplasma gondii, TGME49_305340, TgHDAC3, nanoLC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Cout��
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD016845

PRIDE project URI

• PRIDE
  1. PXD016845
     1. Label: PRIDE project
     2. Name: MS-based quantitative proteomic analysis of total proteomes from T. gondii strains upon MORC���s depletion and TgHDAC3 inhibition.