PXD016492 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic analysis of cardiac biowires, cardiac hypertrophy patient explants, and hypertrophic mouse heart |
Description | we apply and evaluate an integrative mass spectrometry-based tissue profiling strategy that allows for a global quantitative phospho-proteomic survey of normal and disease tissue from human, mouse and OOC-derived specimens. The applicability and utility of this approach was tested in the context of fibrotic cardiac tissue samples from Biowire OOC specimens versus cardiac tissue surgical explants from hypertrophic patients and a transaortic constriction mouse pressure-overload model. Unique attributes and phosphorylation signatures specific to each tissue source were identified, but the results clearly showed that clinically-actionable biological inferences are generated by leveraging commonalities exhibited across the compendium. To validate the application of the cross-platform analytical framework, proof of principle drug testing was performed for selection of anti-fibrotic compounds targeting one of the identified fibrosis-related kinases, GSK3, consistent with a role as a key mediator of fibrosis. |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-15 |
AnnouncementXML | Submission_2020-07-15_08:37:41.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uros Kuzmanov |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-11-28 02:42:37 | ID requested | |
⏵ 1 | 2020-07-15 08:37:41 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Phosphoproteomic, cardiovascular, TMT, LC-MS |
Contact List
Andrew Emili |
contact affiliation | 9Departments of Biochemistry and Biology and the Centre for Network Systems Biology, Boston University School of Medicine, Boston, MA, United States |
contact email | aemili@bu.edu |
lab head | |
Uros Kuzmanov |
contact affiliation | University of Toronto |
contact email | uros.kuzmanov@utoronto.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD016492
- Label: PRIDE project
- Name: Phosphoproteomic analysis of cardiac biowires, cardiac hypertrophy patient explants, and hypertrophic mouse heart