PXD016492-1

PXD016492 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis of cardiac biowires, cardiac hypertrophy patient explants, and hypertrophic mouse heart
Description	we apply and evaluate an integrative mass spectrometry-based tissue profiling strategy that allows for a global quantitative phospho-proteomic survey of normal and disease tissue from human, mouse and OOC-derived specimens. The applicability and utility of this approach was tested in the context of fibrotic cardiac tissue samples from Biowire OOC specimens versus cardiac tissue surgical explants from hypertrophic patients and a transaortic constriction mouse pressure-overload model. Unique attributes and phosphorylation signatures specific to each tissue source were identified, but the results clearly showed that clinically-actionable biological inferences are generated by leveraging commonalities exhibited across the compendium. To validate the application of the cross-platform analytical framework, proof of principle drug testing was performed for selection of anti-fibrotic compounds targeting one of the identified fibrosis-related kinases, GSK3, consistent with a role as a key mediator of fibrosis.
HostingRepository	PRIDE
AnnounceDate	2020-07-15
AnnouncementXML	Submission_2020-07-15_08:37:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Uros Kuzmanov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-11-28 02:42:37	ID requested
⏵ 1	2020-07-15 08:37:41	announced

Dataset with its publication pending

submitter keyword: Phosphoproteomic, cardiovascular, TMT, LC-MS

Andrew Emili
contact affiliation	9Departments of Biochemistry and Biology and the Centre for Network Systems Biology, Boston University School of Medicine, Boston, MA, United States
contact email	aemili@bu.edu
lab head
Uros Kuzmanov
contact affiliation	University of Toronto
contact email	uros.kuzmanov@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD016492

PRIDE project URI

• PRIDE
  1. PXD016492
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis of cardiac biowires, cardiac hypertrophy patient explants, and hypertrophic mouse heart