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PXD016323 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS analysis of human long ribosomal RNA
DescriptionThe field of epitranscriptomics is growing in importance, with chemical modification of RNA being associated with a wide variety of biological phenomena. Mass spectrometry (MS) enables the identification of modified RNA residues within their sequence contexts, by using analogous approaches to shotgun proteomics. We have developed a free and open-source database search engine for RNA MS data, called NucleicAcidSearchEngine (NASE), as part of the OpenMS software framework. NASE allows the reliable identification of (modified) RNA sequences from LC-MS/MS data in a high-throughput fashion. For this validation dataset, we generated samples of human long ribosomal RNA from a cellular extract. The samples were RNase-treated prior to nanoflow LC-MS/MS analysis.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHendrik Weisser
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue; acetylated residue; dimethylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-11-18 07:35:56ID requested
12020-03-31 04:49:02announced
Publication List
Wein S, Andrews B, Sachsenberg T, Santos-Rosa H, Kohlbacher O, Kouzarides T, Garcia BA, Weisser H, A computational platform for high-throughput analysis of RNA sequences and modifications by mass spectrometry. Nat Commun, 11(1):926(2020) [pubmed]
Keyword List
submitter keyword: RNA, rRNA, 18S, 28S, epitranscriptomics
Contact List
Byron Andrews
contact affiliationSTORM Therapeutics Limited, Cambridge, United Kingdom
contact emailbyron.andrews@stormtherapeutics.com
lab head
Hendrik Weisser
contact affiliationSTORM Therapeutics Limited
contact emailhendrik.weisser@stormtherapeutics.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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