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PXD015871 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePotential interactors of alpha-tubulin acetyltransferase 1 (αTAT1/MEC17)
DescriptionCell adhesion to the extracellular matrix occurs through integrin-mediated focal adhesions, which sense the mechanical properties of the substrate and impact cellular functions such as cell migration. Mechanotransduction at focal adhesions affects the actomyosin network and contributes to cell migration. Despite being key players in cell adhesion and migration, the role of microtubules in mechanotransduction has been overlooked. Here, we show that substrate rigidity increases microtubule acetylation through β1 integrin signalling in primary rat astrocytes. Moreover, αTAT1, the enzyme responsible for microtubule acetylation, interacts with a major mechanosensing focal adhesion protein, Talin, and is able to tune the distribution of focal adhesions depending on the matrix rigidity. αTAT1 also reorganises the actomyosin network, increases traction force generation and cell migration speed on stiff substrates. Mechanistically, acetylation of microtubules promotes the release of microtubule-associated RhoGEF, GEF-H1 into the cytoplasm, which then leads to RhoA activation and high actomyosin contractility. Thus, we propose a novel feedback loop involving a crosstalk between microtubules and actin in mechanotransduction at focal adhesions whereby, cells sense the rigidity of the substrate through integrin-mediated adhesions, modulate their levels of microtubule acetylation, which then controls the actomyosin cytoskeleton and force transmission on the substrate to promote mechanosensitive cell migration.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterValentin SABATET
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-10-17 02:57:58ID requested
12021-11-02 04:27:41announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Homo sapiens, HEK cells, αTAT1, MEC17
Contact List
Damarys Loew
contact affiliationHead of the Curie Institute Mass Spectrometry Platform
contact emaildamarys.loew@curie.fr
lab head
Valentin SABATET
contact affiliationCurie Institute
contact emailvalentin.sabatet@curie.fr
dataset submitter
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Dataset FTP location
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PRIDE project URI
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