Updated publication reference for PubMed record(s): 32184246. Drastic alterations in transcripts associated with central carbon metabolism and associatedprocesses have been repeatedly observed a common expression program as P. aeruginosa adapts to the cystic fibrosis lung when compared to standard laboratory conditions. However, we have limited knowledge of how well these transcriptomic changes correlate with actual alterations in metabolic flux; the rate of turnover of molecules through metabolic pathways. We have compared the proteome of Pseudomonas aeruginosa PAO1 during growth on the carbon sources acetate and glycerol.