PXD015599 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of Bona Fide Targets of AMPylating Fic-enzymes by Substrate Mediated Covalent Capture |
Description | Many pathogenic bacteria utilize posttranslational modifications to manipulate central components of the host cell functions. Often, the released enzymes belong to the large Fic-family, causing target modification with nucleotide monophosphates. Due to the lack of a generic method for the identification of the Fic-family’s cellular targets, we have established a new approach by combining synthetic thiol-reactive nucleotide derivatives (TReNDs) with recombinantly produced Fic-enzymes containing strategically placed cysteines in their active sites, thereby producing reactive binary FicTReND probes for covalent substrate capture. The binary probes successfully capture their targets from complex lysates and permit their identification by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2020-07-09 |
AnnouncementXML | Submission_2020-07-09_02:17:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christoph Krisp |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-09-26 01:55:50 | ID requested | |
⏵ 1 | 2020-07-09 02:17:41 | announced | |
2 | 2024-10-22 05:08:45 | announced | 2024-10-22: Updated project metadata. |
Publication List
Gulen B, Rosselin M, Fauser J, Albers MF, Pett C, Krisp C, Pogenberg V, Schl, ü, ter H, Hedberg C, Itzen A, Identification of targets of AMPylating Fic enzymes by co-substrate-mediated covalent capture. Nat Chem, 12(8):732-739(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Post-translational modifications, AMPylation, Fic enzymes, Host-pathogen interactions |
Contact List
Azmelt tyen |
contact affiliation | University Medical Center Hamburg Eppendorf Center for Experimental Medicine, Institute of Biochemisry and Signaltransduction Hamburg Germany |
contact email | a.itzen@uke.de |
lab head | |
Christoph Krisp |
contact affiliation | University Medical Center Hamburg-Eppendorf Institute of Clinical Chemistry and Laboratory Medicine Mass Spectrometric Proteomics |
contact email | c.krisp@uke.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD015599
- Label: PRIDE project
- Name: Identification of Bona Fide Targets of AMPylating Fic-enzymes by Substrate Mediated Covalent Capture