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PXD015255

PXD015255 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman neuronal extracellular vesicles contain a transcriptional network and tetraspanin-defined subpopulations
DescriptionHealthy brain function is mediated by several complementary signalling pathways, many of which are driven by extracellular vesicles (EVs). EVs are heterogeneous in both size and cargo and are constitutively released from cells into the extracellular milieu. They are subsequently trafficked to recipient cells, whereupon their entry can modify the cellular phenotype. Here, in order to further understand the functional role of EVs in neurons, we isolated EVs by size exclusion chromatography from human induced pluripotent stem cell (iPSC)-derived neurons. Electron microscopy and dynamic light scattering revealed that the isolated EVs had a diameter of 30-100 nm. Transcriptomic and proteomics analyses of the EVs and neurons identified key molecules enriched in the EVs involved in cell surface interaction (integrins and collagens), internalisation pathways (clathrin- and caveolin-dependent), downstream signalling pathways (phospholipases, integrin-linked kinase and MAPKs), and long-term impacts on cellular development and maintenance. Overall, we show that key signalling networks and mechanisms are enriched in EVs isolated from human iPSC-derived neurons, and identify subpopulations of EVs defined by differential tetraspanin expression.
HostingRepositoryPRIDE
AnnounceDate2021-09-08
AnnouncementXMLSubmission_2021-09-08_14:04:42.704.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Hicks
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-08-30 08:34:37ID requested
12021-09-08 14:04:43announced
Publication List
Hicks DA, Jones AC, Corbett NJ, Fisher K, Pickering-Brown SM, Ashe MP, Hooper NM, Extracellular Vesicles Isolated from Human Induced Pluripotent Stem Cell-Derived Neurons Contain a Transcriptional Network. Neurochem Res, 45(7):1711-1728(2020) [pubmed]
Keyword List
submitter keyword: Human, neuron, iPSC, LC-MS/MS
Contact List
David Hicks
contact affiliationUniversity of Manchester
contact emaildavid.hicks-2@manchester.ac.uk
lab head
David Hicks
contact affiliationUniversity of Manchester
contact emaildavid.hicks-2@manchester.ac.uk
dataset submitter
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Dataset FTP location
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