Updated project metadata. The Mycobacterium tuberculosis eukaryotic-like serine/threonine protein kinase (STPKs) PknA and PknB regulate several processes required for cell growth, both kinase have been found to be and can be investigated by using the conditional mutant. In the present study, we created the M. tuberculosis kinase PknA/ PknB depletion mutant and performed the phosphoproteomics to uncover phosphorylation substrates changes when PknA/ PknB depletion.