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PXD014845 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal analysis of the human cytomegalovirus interactome identifies degradation hubs, domain associations and viral protein functions
DescriptionHuman cytomegalovirus (HCMV) is an important pathogen that extensively modulates host cells, downregulating >900 human proteins over the course of viral replication and degrading ≥133 proteins shortly after infection. The mechanism of degradation of most host proteins remains unresolved, and the functions of many viral proteins are incompletely characterised. We performed a systematic interactome analysis of 169 canonical HCMV proteins, and a subset of non-canonical HCMV proteins, in infected cells. This identified an extensive network of >3,400 virus-host and >150 virus-virus protein interactions, providing insights into novel functions for multiple viral genes. Domain analysis predicted binding of the viral UL25 protein to the SH3 domains of NCK Adaptor Protein 1. Viral interacting proteins were identified for 31/133 degraded host targets. Finally, the uncharacterised, non-canonical ORFL147C protein was found to interact with elements of the mRNA splicing machinery, and a mutational study suggested its importance in viral replication. The interactome data will be important for future studies of herpesvirus infection.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichael Weekes
SpeciesList scientific name: Human herpesvirus 5 strain Merlin; NCBI TaxID: 295027; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-31 01:24:03ID requested
12020-01-22 05:40:01announced
Publication List
Nobre LV, Nightingale K, Ravenhill BJ, Antrobus R, Soday L, Nichols J, Davies JA, Seirafian S, Wang EC, Davison AJ, Wilkinson GW, Stanton RJ, Huttlin EL, Weekes MP, Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions. Elife, 8():(2019) [pubmed]
Keyword List
submitter keyword: proteomics
systems virology
human cytomegalovirus
host-pathogen interaction
immune evasion
protein-protein interaction
Contact List
Michael Weekes
contact affiliationCambridge Institute for Medical Research, University of Cambridge, CB2 0XY, UK
contact emailmpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliationUniversity of Cambridge
contact emailmpw1001@cam.ac.uk
dataset submitter
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Dataset FTP location
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