PXD014311

PXD014311 is an original dataset announced via ProteomeXchange.

Title	Region-specific proteome changes of the intestinal epithelium during aging and dietary restriction
Description	The small intestine is responsible for nutrient absorption and it is one of the most important interfaces between the environment and our body. During aging, changes in the structure of the epithelium lead to food malabsorption and reduced barrier function thus increasing disease risk in aging. The molecular drivers of these alterations remain poorly understood. Here, we compared the proteomes of small intestinal crypts from mice across different anatomical regions and age groups. We found that aging alters epithelial immune responses, metabolic networks and stem cell proliferation, and it is accompanied by a region-dependent skewing in the cellular composition of the intestinal epithelium. Of note, a short period of dietary restriction followed by re-feeding partially restores the epithelium to a youthful state by promoting the differentiation of intestinal stem cells (ISCs) towards the secretory lineage. Using in vitro and in vivo studies, we identify Hmgcs2 (3-hydroxy-3-methylglutaryl-CoA synthetase 2) – the rate limiting enzyme in the synthesis of ketone bodies – as a modulator of ISCs differentiation, which responds to dietary changes. This study provides an atlas of age-dependent proteome changes in defined regions of the intestinal epithelium and characterizes how young and old mice adapt to drastic changes of diet, such as dietary restriction and re-feeding.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:54.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Ori
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-20 02:02:46	ID requested
1	2020-04-29 02:04:27	announced
2	2020-05-06 00:51:47	announced	2020-05-06: Updated publication reference for PubMed record(s): 32348758.
⏵ 3	2024-10-22 05:02:56	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2020.107565;

Gebert N, Cheng CW, Kirkpatrick JM, Di Fraia D, Yun J, Sch, ä, del P, Pace S, Garside GB, Werz O, Rudolph KL, Jasper H, Yilmaz Ö H, Ori A, Region-Specific Proteome Changes of the Intestinal Epithelium during Aging and Dietary Restriction. Cell Rep, 31(4):107565(2020) [pubmed]

submitter keyword: stem cell, crypts, diet, small intestine,Aging

Alessandro Ori
contact affiliation	Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Jena, Germany
contact email	alessandro.ori@leibniz-fli.de
lab head
Alessandro Ori
contact affiliation	Leibniz Institute on Aging
contact email	aleori@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD014311

PRIDE project URI

• PRIDE
  1. PXD014311
     1. Label: PRIDE project
     2. Name: Region-specific proteome changes of the intestinal epithelium during aging and dietary restriction