PXD014175

PXD014175 is an original dataset announced via ProteomeXchange.

Title	A chemical proteomic analysis of illudin-interacting proteins
Description	The illudin natural product family are fungal secondary metabolites with a characteristic spirocyclopropyl-substituted fused 6,5-bicyclic ring system. They have been extensively studied for their cytotoxicity in various tumor cell types and semisynthetic derivatives with improved therapeutic characteristics have progressed into clinical trials. While it is believed that this potent alkylating compound class is mainly acting via DNA modification, little is known about its binding to protein sites in a cellular context. In order to reveal putative protein targets of the illudin family in live cancer cells, we employed a semisynthetic strategy to access a series of illudin-based probes for activity-based protein profiling (ABPP). While the probes largely retained potent cytotoxicity, proteomic profiling studies unravelled multiple protein hits suggesting that illudins exert their mode of action not from addressing a specific protein target but rather from DNA modification and unselective protein binding.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:55:47.565.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nina Bach
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-07 04:35:25	ID requested
1	2020-01-09 05:19:34	announced
⏵ 2	2024-10-22 04:55:50	announced	2024-10-22: Updated project metadata.

10.1002/chem.201902919;

Le P, Nodwell MB, Eirich J, Sieber SA, A Chemical Proteomic Analysis of Illudin-Interacting Proteins. Chemistry, 25(54):12644-12651(2019) [pubmed]

curator keyword: Biological

submitter keyword: illudins,Activity based protein profiling

Stephan A. Sieber
contact affiliation	TU Muenchen Department of Chemistry Organic Chemistry II
contact email	stephan.sieber@tum.de
lab head
Nina Bach
contact affiliation	TU München
contact email	nina.bach@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014175

PRIDE project URI

• PRIDE
  1. PXD014175
     1. Label: PRIDE project
     2. Name: A chemical proteomic analysis of illudin-interacting proteins