To establish the protein expression profile of Ba168, a high-resolution LC-MS/MS proteomic analysis was performed. A total of 1155 proteins were identified from 5233 unique peptides. GO and KEGG analysis revealed that a majority of the proteins were associated with biosynthesis and carbon metabolism pathways, such as biosynthesis of amino acids, peptidoglycan, and antibiotics. Then, we identified the antimicrobial proteins of Ba168. At least 16 potential antimicrobial-activity-related proteins were identified; 11 of these proteins have direct antimicrobial effects, while 5 of these proteins are associated with the formation of antimicrobial substances.